by,on,through,with,in 区别如下

【1】by “通过” 主要强调某事以某种特定方式完成某事(英文解释：If you do something by a particular means, you do it using that thing） 

The house is heated by gas.这房子是煤气供暖的。

May I pay by cheque ？我能用支票付款吗？

I will contact you by letter.我会给你写信联系的。

Switch it on by pressing this button.按下这个开关启动它

Tom likes travelling by plane汤姆喜欢乘坐飞机旅行。

【2】on  “通过；使用”借助于 主要强调使用或者借助于某种工具完成某种动作行为((英文解释If something is done on an instrument or a machine, it is done using that instrument or machine.）

She played a tune on her guitar.她用她的吉他弹了一支曲子。

The information is available on the Internet.从互联网上可得到这个信息。

We spoke on the phone.我们通过电话谈了谈。

What's on TV?电视上有什么节目？

The programme's on Channel 4.这个节目在4频道。

【3】through “通过;凭借;依靠” 强调依靠某种方式来完成某事(英文解释：You use through when stating the means by which a particular thing is achieved.）

Those who seek to grab power through violence deserve punishment.企图通过暴力夺权的人理应受到惩罚。

You simply can't get a ticket through official channels.通过官方渠道是压根儿搞不到票的。

You can only achieve success through hard work.你得孜孜不倦方能成功。

The accident happened through no fault of mine.发生事故并不是我的过错。

【4】with “用,使用”(某种工具、物品或材料 强调用某种具体用具做某事(英文解释：If you do something with a particular tool, object, or substance, you do it using that tool, object, or substance.）

Remove the meat with a fork and divide it among four plates.  用餐叉把肉叉走，分到4个盘里。

Pack the fruits and nuts into the jars and cover with brandy.把水果和坚果放进罐子里，用白兰地酒浸泡。

Cut it with a knife.用刀把它切开。

It is treated with acid before being analysed.对它先用酸处理再进行分析。

【5】in “以…方式”强调某人以何种方式去表达某事(英文解释：You use in to indicate how someone is expressing something.）

 Information is given to the patient verbally and in writing.  用口头和书面的形式向病人提供信息。

He likes lessons in languages other than Spanish.  他喜欢用西班牙语以外的语言讲授的课程

Tom can't say it in English.汤姆不会用英语说它。

She wrote in pencil.她用铅笔写的。

Put it in writing.把它写下来。

I paid in cash.我用现金支付的。

He spoke in a loud voice.他大声说话。