Was the letter written with a pen? (pen 指钢笔)
Was the letter written in pen? (pen 指墨水)
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可以。
Please fill out the form in pen.请用钢笔填写此表。(摘自2019年出版的《朗文当代高级英语辞典》第六版)
此句可以改为:Please fill out the form with a pen.
in pen指“色彩”,而非“工具”,如下句中的in pencil就是指“铅笔字”。
That letter was written in pencil. 那封信是用铅笔写的。《现代汉英综合大词典》